Enhanced transgene expression from single-stranded AAV vectors in human cells in vitro and in murine hepatocytes in vivo

• Main Authors: Yuan Lu (Author), Chen Ling (Author), Jakob Shoti (Author), Hua Yang (Author), Aneesha Nath (Author), Geoffrey D. Keeler (Author), Keyun Qing (Author), Arun Srivastava (Author)
• Format: Book
• Published: Elsevier, 2024-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We identified that distal 10 nucleotides in the D-sequence in AAV2 inverted terminal repeat (ITR) share partial sequence homology to 1/2 binding site of glucocorticoid receptor-binding element (GRE). Here, we describe that (1) purified GR binds to AAV2 D-sequence, and the D-sequence competes with GR...