Purification and Biochemical Characterization of Taxadiene Synthase from <i>Bacillus koreensis</i> and <i>Stenotrophomonas maltophilia</i>

• Main Authors: Ashraf S. A. El-Sayed (Author), Maher Fathalla (Author), Ahmed A. Shindia (Author), Amgad M. Rady (Author), Ashraf F. El-Baz (Author), Yara Morsy (Author), Basel Sitohy (Author), Mahmoud Sitohy (Author)
• Format: Book
• Published: MDPI AG, 2021-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Taxadiene synthase (TDS) is the rate-limiting enzyme of Taxol biosynthesis that cyclizes the geranylgeranyl pyrophosphate into taxadiene. Attenuating Taxol productivity by fungi is the main challenge impeding its industrial application; it is possible that silencing the expression of TDS is the most...