Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats

• Main Authors: Kohei TORIKAI (Author), Kazuma SHIMIZU (Author), Hiroaki NAGATOMO (Author), Mariko KASAI (Author), Megumi KATO-ITOH (Author), Yuko KAMADA (Author), Ikue SHIBASAKI (Author), Hyojung JEON (Author), Riko KIKUCHI (Author), Sayaka WAKAYAMA (Author), Fabian SUCHY (Author), Hiromitsu NAKAUCHI (Author), Teruhiko WAKAYAMA (Author), Eiji MIZUTANI (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. First, the methods for artificially removing the mouse sperm tail were evaluated. Trypsin treatment was found to...