Multiple-Monitor HPLC Assays for Rapid Process Development, In-Process Monitoring, and Validation of AAV Production and Purification

• Main Authors: Pete Gagnon (Author), Blaz Goricar (Author), Nina Mencin (Author), Timotej Zvanut (Author), Sebastijan Peljhan (Author), Maja Leskovec (Author), Ales Strancar (Author)
• Format: Book
• Published: MDPI AG, 2021-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

HPLC is established as a fast convenient analytical technology for characterizing the content of empty and full capsids in purified samples containing adeno-associated virus (AAV). UV-based monitoring unfortunately over-estimates the proportion of full capsids and offers little value for characteriz...