FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells

• Main Authors: Fang He (Author), Na Ni (Author), Zongyue Zeng (Author), Di Wu (Author), Yixiao Feng (Author), Alexander J. Li (Author), Benjamin Luu (Author), Alissa F. Li (Author), Kevin Qin (Author), Eric Wang (Author), Xi Wang (Author), Xiaoxing Wu (Author), Huaxiu Luo (Author), Jing Zhang (Author), Meng Zhang (Author), Yukun Mao (Author), Mikhail Pakvasa (Author), William Wagstaff (Author), Yongtao Zhang (Author), Changchun Niu (Author), Hao Wang (Author), Linjuan Huang (Author), Deyao Shi (Author), Qing Liu (Author), Xia Zhao (Author), Kai Fu (Author), Russell R. Reid (Author), Jennifer Moriatis Wolf (Author), Michael J. Lee (Author), Kelly Hynes (Author), Jason Strelzow (Author), Mostafa El Dafrawy (Author), Hua Gan (Author), Tong-Chuan He (Author), Jiaming Fan (Author)
• Format: Book
• Published: Elsevier, 2020-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

RNA interference (RNAi) is mediated by an ∼21-nt double-stranded small interfering RNA (siRNA) and shows great promise in delineating gene functions and in developing therapeutics for human diseases. However, effective gene silencing usually requires the delivery of multiple siRNAs for a given gene,...