Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

• Κύριοι συγγραφείς: Yong Ma (Συγγραφέας), Buyun Chen (Συγγραφέας), Donglu Zhang (Συγγραφέας)
• Μορφή: Βιβλίο
• Έκδοση: Elsevier, 2020-06-01T00:00:00Z.
• Θέματα: article
• Διαθέσιμο Online: Connect to this object online.

Accurate DNA quantitation is a prerequisite in many biomedical and pharmaceutical studies. Here we established a new DNA quantitation method by nuclease P1 digestion and UPLC-MS/MS analysis. DNA fragments can be efficiently hydrolyzed to single deoxyribonucleotides by nuclease P1 in a short time. Th...