Rational design of a new mutant of tobacco etch virus protease in order to increase the in vitro solubility

• Main Authors: Hossein Mohammadian (Author), Karim Mahnam (Author), Hamid Mirmohammad Sadeghi (Author), Mohamad Reza Ganjalikhany (Author), Vajihe Akbari (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background and purpose: Tobacco etch virus (TEV) protease is a protease with high sequence specificity which is useful for the cleavage of fusion proteins. A major limitation of this enzyme is its relatively poor solubility. This study aimed to investigate the effects of some suggested mutations by...