Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing

• Main Authors: Emilie Lecomte (Author), Benoît Tournaire (Author), Benjamin Cogné (Author), Jean-Baptiste Dupont (Author), Pierre Lindenbaum (Author), Mélanie Martin-Fontaine (Author), Frédéric Broucque (Author), Cécile Robin (Author), Matthias Hebben (Author), Otto-Wilhelm Merten (Author), Véronique Blouin (Author), Achille François (Author), Richard Redon (Author), Philippe Moullier (Author), Adrien Léger (Author)
• Format: Book
• Published: Elsevier, 2015-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Recent successful clinical trials with recombinant adeno-associated viral vectors (rAAVs) have led to a renewed interest in gene therapy. However, despite extensive developments to improve vector-manufacturing processes, undesirable DNA contaminants in rAAV preparations remain a major safety concern...