The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens.

• Principais autores: Chelsea N Davis (Autor), Helen Phillips (Autor), John J Tomes (Autor), Martin T Swain (Autor), Toby J Wilkinson (Autor), Peter M Brophy (Autor), Russell M Morphew (Autor)
• Formato: Livro
• Publicado em: Public Library of Science (PLoS), 2019-02-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

BACKGROUND:Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-secretory products are necessary for downstream studies and application development. The most widely used purification method of EVs for helminth pathogens is currently differential centrif...