The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens.

• Main Authors: Chelsea N Davis (Author), Helen Phillips (Author), John J Tomes (Author), Martin T Swain (Author), Toby J Wilkinson (Author), Peter M Brophy (Author), Russell M Morphew (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:Robust protocols for the isolation of extracellular vesicles (EVs) from the rest of their excretory-secretory products are necessary for downstream studies and application development. The most widely used purification method of EVs for helminth pathogens is currently differential centrif...