Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair

• Main Authors: Kankan Wang (Author), Xiaochun Tang (Author), Yan Liu (Author), Zicong Xie (Author), Xiaodong Zou (Author), Mengjing Li (Author), Hongming Yuan (Author), Hongsheng Ouyang (Author), Huping Jiao (Author), Daxin Pang (Author)
• Format: Book
• Published: Elsevier, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Precise genome editing in livestock is of great value for the fundamental investigation of disease modeling. However, genetically modified pigs carrying subtle point mutations were still seldom reported despite the rapid development of programmable endonucleases. Here, we attempt to investigate sing...