Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface in vitro

• Main Authors: Ludovica Parisi (Author), Massimiliano Giovanni Bianchi (Author), Benedetta Ghezzi (Author), Eleonora Maurizi (Author), Guido Maria Macaluso (Author), Ovidio Bussolati (Author), Simone Lumetti (Author)
• Format: Book
• Published: Elsevier, 2023-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background/purpose: Testing of dental materials when in contact with innate immune cells has been so far hindered by the lack of proper in vitro models. Human primary monocyte-derived macrophages (MDMs) would be an excellent option to this aim. However, the inability to detach them from the tissue c...