CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments

• Main Authors: Annekatrien Boel (Author), Hanna De Saffel (Author), Wouter Steyaert (Author), Bert Callewaert (Author), Anne De Paepe (Author), Paul J. Coucke (Author), Andy Willaert (Author)
• Format: Book
• Published: The Company of Biologists, 2018-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Targeted genome editing by CRISPR/Cas9 is extremely well fitted to generate gene disruptions, although precise sequence replacement by CRISPR/Cas9-mediated homology-directed repair (HDR) suffers from low efficiency, impeding its use for high-throughput knock-in disease modeling. In this study, we us...