Improving Editing Efficiency for the Sequences with NGH PAM Using xCas9-Derived Base Editors

• Main Authors: Xinyi Liu (Author), Guanglei Li (Author), Xueliang Zhou (Author), Yunbo Qiao (Author), Ruixuan Wang (Author), Shaohui Tang (Author), Jianqiao Liu (Author), Lisheng Wang (Author), Xingxu Huang (Author)
• Format: Book
• Published: Elsevier, 2019-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The development of CRISPR/Cas9-mediated base editors (BEs) provided a versatile tool for precise genome editing. The recently developed xCas9-derived base editors (xBEs) that recognize the NG PAM substantially expand the targeting scope in the genome, while their editing efficiency needs to be impro...