Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos

• Main Authors: Fuminori TANIHARA (Author), Maki HIRATA (Author), Nhien Thi NGUYEN (Author), Quynh Anh LE (Author), Takayuki HIRANO (Author), Takeshige OTOI (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2019-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Cytoplasmic microinjection (CI) of the CRISPR/Cas9 system enabled the induction of site-specific mutations in porcine zygotes and resulting pigs. However, mosaicism is a serious problem for genetically modified pigs. In the present study, we investigated suitable timing and concentration of CRISPR/C...