Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos
Cytoplasmic microinjection (CI) of the CRISPR/Cas9 system enabled the induction of site-specific mutations in porcine zygotes and resulting pigs. However, mosaicism is a serious problem for genetically modified pigs. In the present study, we investigated suitable timing and concentration of CRISPR/C...
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| Main Authors: | , , , , , |
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| Format: | Book |
| Published: |
The Society for Reproduction and Development,
2019-02-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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