Identification of critical chemical modifications by size exclusion chromatography of stressed antibody-target complexes with competitive binding

• Main Authors: Rachel Liuqing Shi (Author), Gang Xiao (Author), Thomas M. Dillon (Author), Arnold McAuley (Author), Margaret S. Ricci (Author), Pavel V. Bondarenko (Author)
• Format: Book
• Published: Taylor & Francis Group, 2021-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Chemical modifications (attributes) in the binding regions of stressed therapeutic proteins may affect binding to target and efficacy of therapeutic proteins. The method presented here describes the criticality assessment of therapeutic antibody modifications by size-exclusion chromatography (SEC) o...