A protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis.

• Main Authors: Kailash P Patra (Author), Mayuko Saito (Author), Vidya L Atluri (Author), Hortensia G Rolán (Author), Briana Young (Author), Tobias Kerrinnes (Author), Henk Smits (Author), Jessica N Ricaldi (Author), Eduardo Gotuzzo (Author), Robert H Gilman (Author), Renee M Tsolis (Author), Joseph M Vinetz (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2014-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Human brucellosis is most commonly diagnosed by serology based on agglutination of fixed Brucella abortus as antigen. Nucleic acid amplification techniques have not proven capable of reproducibly and sensitively demonstrating the presence of Brucella DNA in clinical specimens. We sought to optimize...