Screening of the Pathogen Box for inhibitors with dual efficacy against Giardia lamblia and Cryptosporidium parvum.

• Main Authors: Kelly M Hennessey (Author), Ilse C Rogiers (Author), Han-Wei Shih (Author), Matthew A Hulverson (Author), Ryan Choi (Author), Molly C McCloskey (Author), Grant R Whitman (Author), Lynn K Barrett (Author), Ethan A Merritt (Author), Alexander R Paredez (Author), Kayode K Ojo (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

There is need for a more efficient cell-based assay amenable to high-throughput drug screening against Giardia lamblia. Here, we report the development of a screening method utilizing G. lamblia engineered to express red-shifted firefly luciferase. Parasite growth and replication were quantified usi...