Experimental Conditions That Influence the Utility of 2'7'-Dichlorodihydrofluorescein Diacetate (DCFH₂-DA) as a Fluorogenic Biosensor for Mitochondrial Redox Status

• Main Authors: Lianne R. de Haan (Author), Megan J. Reiniers (Author), Laurens F. Reeskamp (Author), Ali Belkouz (Author), Lei Ao (Author), Shuqun Cheng (Author), Baoyue Ding (Author), Rowan F. van Golen (Author), Michal Heger (Author)
• Format: Book
• Published: MDPI AG, 2022-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Oxidative stress has been causally linked to various diseases. Electron transport chain (ETC) inhibitors such as rotenone and antimycin A are frequently used in model systems to study oxidative stress. Oxidative stress that is provoked by ETC inhibitors can be visualized using the fluorogenic probe...