Non-invasive monitoring of drug action: A new live in vitro assay design for Chagas' disease drug discovery.

• Main Authors: Anna F Fesser (Author), Olivier Braissant (Author), Francisco Olmo (Author), John M Kelly (Author), Pascal Mäser (Author), Marcel Kaiser (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2020-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

New assay designs are needed to improve the predictive value of the Trypanosoma cruzi in vitro tests used as part of the Chagas' disease drug development pipeline. Here, we employed a green fluorescent protein (eGFP)-expressing parasite line and live high-content imaging to monitor the growth o...