Nested PCR and the TaqMan SNP Genotyping Assay enhanced the sensitivity of drug resistance testing of Mycobacterium leprae using clinical specimens of leprosy patients.

• Main Authors: Xiaohua Chen (Author), Jun He (Author), Jian Liu (Author), Yuangang You (Author), Lianchao Yuan (Author), Yan Wen (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:Although leprosy is efficiently treated by multidrug therapy, resistance to first-line (dapsone, rifampin) and second-line (fluoroquinolones) drugs has been described worldwide. However, the characteristics of drug resistance in Southwest China remain unknown. Furthermore, the sensitivity...