LCâMS/MS assay for the determination of natamycin in rabbit and human plasma: Application to a pharmacokinetics and protein binding study
To enable reliable quantification of natamycin in rabbit and human plasma, a validated, sensitive and selective liquid chromatographyâtandem mass spectrometry assay was developed. The chromatographic separation was achieved isocratically on a Cyano column using methanol: aqueous 3.5Â mM ammonium ace...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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Elsevier,
2013-04-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | To enable reliable quantification of natamycin in rabbit and human plasma, a validated, sensitive and selective liquid chromatographyâtandem mass spectrometry assay was developed. The chromatographic separation was achieved isocratically on a Cyano column using methanol: aqueous 3.5Â mM ammonium acetate (pH 4) (90:10Â v/v). The assay was validated over a concentration range of 6.25â400Â ng/mL with lower limit of detection of 3.12Â ng/mL. Quantification was performed using the transitions 664.5â137.2m/z for natamycin and 923.5â183.4m/z for the IS. The method was validated with respect to linearity, accuracy, precision, recovery and stability. This assay has been successfully applied to a pharmacokinetic study of natamycin in NZ rabbit and plasma protein binding in human plasma. Keywords: Natamycin, LCâMS/MS, Pharmacokinetics, Protein binding |
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| Item Description: | 2095-1779 10.1016/j.jpha.2012.11.003 |