LCâMS/MS assay for the determination of natamycin in rabbit and human plasma: Application to a pharmacokinetics and protein binding study
To enable reliable quantification of natamycin in rabbit and human plasma, a validated, sensitive and selective liquid chromatographyâtandem mass spectrometry assay was developed. The chromatographic separation was achieved isocratically on a Cyano column using methanol: aqueous 3.5Â mM ammonium ace...
Na minha lista:
| Main Authors: | , , , , , , |
|---|---|
| Formato: | Livro |
| Publicado em: |
Elsevier,
2013-04-01T00:00:00Z.
|
| Assuntos: | |
| Acesso em linha: | Connect to this object online. |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_7de4f76a2be345a79f18623f5d4e9b23 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Yashpal Singh Chhonker |e author |
| 700 | 1 | 0 | |a Devendra Kumar |e author |
| 700 | 1 | 0 | |a Pankaj Shrivastava |e author |
| 700 | 1 | 0 | |a Deepak Kumar |e author |
| 700 | 1 | 0 | |a Rajbir Singh |e author |
| 700 | 1 | 0 | |a Hardik Chandasana |e author |
| 700 | 1 | 0 | |a Rabi Sankar Bhatta |e author |
| 245 | 0 | 0 | |a LCâMS/MS assay for the determination of natamycin in rabbit and human plasma: Application to a pharmacokinetics and protein binding study |
| 260 | |b Elsevier, |c 2013-04-01T00:00:00Z. | ||
| 500 | |a 2095-1779 | ||
| 500 | |a 10.1016/j.jpha.2012.11.003 | ||
| 520 | |a To enable reliable quantification of natamycin in rabbit and human plasma, a validated, sensitive and selective liquid chromatographyâtandem mass spectrometry assay was developed. The chromatographic separation was achieved isocratically on a Cyano column using methanol: aqueous 3.5Â mM ammonium acetate (pH 4) (90:10Â v/v). The assay was validated over a concentration range of 6.25â400Â ng/mL with lower limit of detection of 3.12Â ng/mL. Quantification was performed using the transitions 664.5â137.2m/z for natamycin and 923.5â183.4m/z for the IS. The method was validated with respect to linearity, accuracy, precision, recovery and stability. This assay has been successfully applied to a pharmacokinetic study of natamycin in NZ rabbit and plasma protein binding in human plasma. Keywords: Natamycin, LCâMS/MS, Pharmacokinetics, Protein binding | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmaceutical Analysis, Vol 3, Iss 2, Pp 144-148 (2013) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2095177912001256 | |
| 787 | 0 | |n https://doaj.org/toc/2095-1779 | |
| 856 | 4 | 1 | |u https://doaj.org/article/7de4f76a2be345a79f18623f5d4e9b23 |z Connect to this object online. |