Robustness of biomarker determination in breast cancer by RT-qPCR: impact of tumor cell content, DCIS and non-neoplastic breast tissue

• Главные авторы: Kerstin Hartmann (Автор), Kornelia Schlombs (Автор), Mark Laible (Автор), Claudia Gürtler (Автор), Marcus Schmidt (Автор), Ugur Sahin (Автор), Hans-Anton Lehr (Автор)
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• Опубликовано: BMC, 2018-10-01T00:00:00Z.
• Предметы: article
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Abstract Background Tissue heterogeneity in formalin-fixed paraffin-embedded (FFPE) breast cancer specimens may affect the accuracy of reverse transcription quantitative real-time PCR (RT-qPCR). Herein, we tested the impact of tissue heterogeneity of breast cancer specimen on the RT-qPCR-based gene...