Development and preliminary evaluation of a multiplexed amplification and next generation sequencing method for viral hemorrhagic fever diagnostics.

• Egile Nagusiak: Annika Brinkmann (Egilea), Koray Ergünay (Egilea), Aleksandar Radonić (Egilea), Zeliha Kocak Tufan (Egilea), Cristina Domingo (Egilea), Andreas Nitsche (Egilea)
• Formatua: Liburua
• Argitaratua: Public Library of Science (PLoS), 2017-11-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

BACKGROUND:We describe the development and evaluation of a novel method for targeted amplification and Next Generation Sequencing (NGS)-based identification of viral hemorrhagic fever (VHF) agents and assess the feasibility of this approach in diagnostics. METHODOLOGY:An ultrahigh-multiplex panel wa...