Development and preliminary evaluation of a multiplexed amplification and next generation sequencing method for viral hemorrhagic fever diagnostics.

• Main Authors: Annika Brinkmann (Author), Koray Ergünay (Author), Aleksandar Radonić (Author), Zeliha Kocak Tufan (Author), Cristina Domingo (Author), Andreas Nitsche (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2017-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:We describe the development and evaluation of a novel method for targeted amplification and Next Generation Sequencing (NGS)-based identification of viral hemorrhagic fever (VHF) agents and assess the feasibility of this approach in diagnostics. METHODOLOGY:An ultrahigh-multiplex panel wa...