Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation

• Main Authors: Koyo Nishimura (Author), Shintaro Fumoto (Author), Yuki Fuchigami (Author), Masayori Hagimori (Author), Kazuo Maruyama (Author), Shigeru Kawakami (Author)
• Format: Book
• Published: Taylor & Francis Group, 2017-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In this study, we demonstrate the low toxicity and highly efficient and spatially improved transfection of plasmid DNA (pDNA) with liposomal nanobubbles (bubble liposomes [BLs]) using ultrasound (US) irradiation in mice. Naked pDNA with BLs was intraperitoneally injected, followed by US irradiation....