Terminal bridging of siRNA duplex at the ribose 2' position controls strand bias and target sequence preference

• Main Authors: Atsushi Shibata (Author), Hisao Shirohzu (Author), Yusuke Iwakami (Author), Tomoaki Abe (Author), Chisato Emura (Author), Eriko Aoki (Author), Tadaaki Ohgi (Author)
• Format: Book
• Published: Elsevier, 2023-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Small interfering RNA (siRNA) and short hairpin RNA (shRNA) are widely used as RNA interference (RNAi) reagents. Recently, truncated shRNAs that trigger RNAi in a Dicer-independent manner have been developed. We generated a novel class of RNAi reagent, designated enforced strand bias (ESB) RNA, in w...