Efficient Transfection of Large Plasmids Encoding HIV-1 into Human Cells-A High Potential Transfection System Based on a Peptide Mimicking Cationic Lipid

• Main Authors: Christopher Janich (Author), Daniel Ivanusic (Author), Julia Giselbrecht (Author), Elena Janich (Author), Shashank Reddy Pinnapireddy (Author), Gerd Hause (Author), Udo Bakowsky (Author), Andreas Langner (Author), Christian Wölk (Author)
• Format: Book
• Published: MDPI AG, 2020-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

One major disadvantage of nucleic acid delivery systems is the low transfection or transduction efficiency of large-sized plasmids into cells. In this communication, we demonstrate the efficient transfection of a 15.5 kb green fluorescent protein (GFP)-fused HIV-1 molecular clone with a nucleic acid...