Variability in the recognition of distinctive immunofluorescence patterns in different brands of HEp-2 cell slides

• Main Authors: Alessandra Dellavance (Author), Wilson de Melo Cruvinel (Author), Paulo Luiz Carvalho Francescantonio (Author), Cristovão Luis Pitangueira Mangueira (Author), Inês Cristina Drugowick (Author), Silvia Helena Rodrigues (Author), Luis Eduardo Coelho Andrade (Author)
• Format: Book
• Published: Sociedade Brasileira de Patologia Clínica, 2013-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

INTRODUCTION: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for the detection of autoantibodies against cellular antigens. However, the culture conditions, cell fixation and permeabilization processes interfere directly in the preservation and spatial distribution of ant...