Engineering the Escherichia coli Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System

• Main Authors: Abigail V. Sharrock (Author), Sarah P. McManaway (Author), Michelle H. Rich (Author), Jeff S. Mumm (Author), Ian F. Hermans (Author), Moana Tercel (Author), Frederik B. Pruijn (Author), David F. Ackerley (Author)
• Format: Book
• Published: Frontiers Media S.A., 2021-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Bacterial nitroreductase enzymes that can efficiently convert nitroaromatic prodrugs to a cytotoxic form have numerous applications in targeted cellular ablation. For example, the generation of cytotoxic metabolites that have low bystander potential (i.e., are largely confined to the activating cell...