Identification of a CE-SDS shoulder peak as disulfide-linked fragments from common CH2 cleavages in IgGs and IgG-like bispecific antibodies

• Үндсэн зохиолчид: Mingyan Cao (Зохиогч), Yang Jiao (Зохиогч), Conner Parthemore (Зохиогч), Samuel Korman (Зохиогч), Jiao Ma (Зохиогч), Alan Hunter (Зохиогч), Greg Kilby (Зохиогч), Xiaoyu Chen (Зохиогч)
• Формат: Ном
• Хэвлэсэн: Taylor & Francis Group, 2021-01-01T00:00:00Z.
• Нөхцлүүд: article
• Онлайн хандалт: Connect to this object online.

Fragmentation is a well-characterized degradation pathway of therapeutic antibodies and is usually monitored by capillary electrophoresis-sodium dodecyl sulfate (CE-SDS). Although fragments due to cleavage in CH2 domains linked by intrachain disulfide bonds are common and can be detected by reduced...