Development of Antibody-Oligonucleotide Complexes for Targeting Exosomal MicroRNA

• Main Authors: Asako Yamayoshi (Author), Shota Oyama (Author), Yusuke Kishimoto (Author), Ryo Konishi (Author), Tsuyoshi Yamamoto (Author), Akio Kobori (Author), Hiroshi Harada (Author), Eishi Ashihara (Author), Hiroshi Sugiyama (Author), Akira Murakami (Author)
• Format: Book
• Published: MDPI AG, 2020-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

MicroRNAs in exosomes (exosomal miRNAs) are considered as significant targets for cancer therapy. Anti-miR oligonucleotides are often used for the functional inhibition of miRNAs; however, there are no studies regarding the regulation of exosomal miRNA functions. In this study, we attempted to develop a novel drug delivery system using anti-exosome antibody-anti-miR oligonucleotide complexes (ExomiR-Tracker) to hijack exosomes to carry anti-miR oligonucleotides inside exosome-recipient cells. We found that ExomiR-Tracker bound to the exosomes, and then the complexes were introduced into the recipient cells. We also found that anti-miR oligonucleotides introduced into the recipient cells can exhibit inhibitory effects on exosomal miRNA functions in vitro and in vivo. We believe that our strategy would be a promising one for targeting exosomal miRNAs.

Item Description

10.3390/pharmaceutics12060545
1999-4923