Application of a ddRT-PCR to quantify seasonal influenza virus for viral isolation

• मुख्य लेखकों: Yimeng Liu (लेखक), Jiachen Zhao (लेखक), Xiaomin Peng (लेखक), Guilan Lu (लेखक), Weixian Shi (लेखक), Zhaomin Feng (लेखक), Hui Xu (लेखक), Shujuan Cui (लेखक), Yang Pan (लेखक), Daitao Zhang (लेखक), Peng Yang (लेखक), Quanyi Wang (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: Elsevier, 2022-10-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.

Viral isolation in cell cultures has been regarded for decades as the "gold standard" for the laboratory diagnosis of influenza viral infections. Not all viral strains could be isolated from clinical samples. This study aimed to quantify the viral load in the samples before isolation to sa...