Application of a ddRT-PCR to quantify seasonal influenza virus for viral isolation

• Main Authors: Yimeng Liu (Author), Jiachen Zhao (Author), Xiaomin Peng (Author), Guilan Lu (Author), Weixian Shi (Author), Zhaomin Feng (Author), Hui Xu (Author), Shujuan Cui (Author), Yang Pan (Author), Daitao Zhang (Author), Peng Yang (Author), Quanyi Wang (Author)
• Format: Book
• Published: Elsevier, 2022-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Viral isolation in cell cultures has been regarded for decades as the "gold standard" for the laboratory diagnosis of influenza viral infections. Not all viral strains could be isolated from clinical samples. This study aimed to quantify the viral load in the samples before isolation to sa...