Mini-PE, a prime editor with compact Cas9 and truncated reverse transcriptase

• Main Authors: Ting Lan (Author), Huangyao Chen (Author), Chengcheng Tang (Author), Yuhui Wei (Author), Yang Liu (Author), Jizeng Zhou (Author), Zhenpeng Zhuang (Author), Quanjun Zhang (Author), Min Chen (Author), Xiaoqing Zhou (Author), Yue Chi (Author), Jinling Wang (Author), Yu He (Author), Liangxue Lai (Author), Qingjian Zou (Author)
• Format: Book
• Published: Elsevier, 2023-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Prime editor (PE) is a versatile genome editing tool that does not need extra DNA donors or inducing double-strand breaks. However, in vivo implementation of PE remains a challenge because of its oversized composition. In this study, we screened out the smallest truncated Moloney murine leukemia vir...