CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

• मुख्य लेखकों: Kevin M. Bloh (लेखक), Pawel A. Bialk (लेखक), Anilkumar Gopalakrishnapillai (लेखक), E. Anders Kolb (लेखक), Eric B. Kmiec (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: Elsevier, 2017-06-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.

Using a CRISPR/Cas9 system, we have reengineered a translational start site in the GATA1 gene in K562 cells. This mutation accounts largely for the onset of myeloid leukemia in Down syndrome (ML-DS). For this reengineering, we utilized CRISPR/Cas9 to generate mammalian cell lines that express trunca...