CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

• Główni autorzy: Kevin M. Bloh (Autor), Pawel A. Bialk (Autor), Anilkumar Gopalakrishnapillai (Autor), E. Anders Kolb (Autor), Eric B. Kmiec (Autor)
• Format: Książka
• Wydane: Elsevier, 2017-06-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.

Using a CRISPR/Cas9 system, we have reengineered a translational start site in the GATA1 gene in K562 cells. This mutation accounts largely for the onset of myeloid leukemia in Down syndrome (ML-DS). For this reengineering, we utilized CRISPR/Cas9 to generate mammalian cell lines that express trunca...