A whole blood intracellular cytokine assay optimised for field site studies demonstrates polyfunctionality of CD4+ T cells in acute scrub typhus.

• Main Authors: Manutsanun Inthawong (Author), Nattapon Pinthong (Author), Areerat Thaiprakhong (Author), Tri Wangrangsimakul (Author), Piyanate Sunyakumthorn (Author), Jennifer Hill (Author), Piengchan Sonthayanon (Author), Daniel H Paris (Author), Susanna J Dunachie (Author), Barbara Kronsteiner (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>Assessment of cellular immune responses by combining intracellular cytokine staining and immunophenotyping using flow cytometry enables the simultaneous measurement of T cell phenotype and effector function in response to pathogens and vaccines. The use of whole blood...