Evaluation of recombinant human interferon beta 1b by liquid chromatography methods and bioassay

• Main Authors: Francielle Santos da Silva (Author), Maurício Elesbão Walter (Author), Bruna Xavier (Author), Rafaela Ferreira Perobelli (Author), Guilherme Zanini Calegari (Author), Douglas Franco Cardoso (Author), Valquiria Guedes Perlin (Author), Sérgio Luiz Dalmora (Author)
• Format: Book
• Published: Universidade de São Paulo, 2019-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Recombinant human interferon beta 1b (rhIFNβ-1b) is clinically used to treat multiple sclerosis. A reversed-phase liquid chromatography (RP-LC) method was carried out on a Jupiter C4 column (250 mm × 4.6 mm i.d.). The mobile phase A consisted of 0.1% trifluoroacetic acid (TFA) in water, and the mobi...