Improvement of developmental competence of cloned male pig embryos by short hairpin ribonucleic acid (shRNA) vector-based but not small interfering RNA (siRNA)-mediated RNA interference (RNAi) of Xist expression

• Main Authors: Xuqiong YANG (Author), Xiao WU (Author), Yang YANG (Author), Ting GU (Author), Linjun HONG (Author), Enqin ZHENG (Author), Zheng XU (Author), Fang ZENG (Author), Junsong SHI (Author), Rong ZHOU (Author), Gengyuan CAI (Author), Zhenfang WU (Author), Zicong LI (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2019-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Xist is an X-linked ribonucleic acid (RNA) gene responsible for the cis induction of X chromosome inactivation (XCI). In cloned mammalian embryos, Xist is ectopically activated at the morula to blastocyst stage on the X chromosome that is supposed to be active, thus resulting in abnormal XCI. Suppre...