Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries

• Main Authors: Michael H. Ronzetti (Author), Bolormaa Baljinnyam (Author), Zina Itkin (Author), Sankalp Jain (Author), Ganesha Rai (Author), Alexey V. Zakharov (Author), Utpal Pal (Author), Anton Simeonov (Author)
• Format: Book
• Published: Frontiers Media S.A., 2022-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Differential scanning fluorimetry is a rapid and economical biophysical technique used to monitor perturbations to protein structure during a thermal gradient, most often by detecting protein unfolding events through an environment-sensitive fluorophore. By employing an NTA-complexed fluorophore tha...