Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries
Differential scanning fluorimetry is a rapid and economical biophysical technique used to monitor perturbations to protein structure during a thermal gradient, most often by detecting protein unfolding events through an environment-sensitive fluorophore. By employing an NTA-complexed fluorophore tha...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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Frontiers Media S.A.,
2022-11-01T00:00:00Z.
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