Theoretical and Experimental Considerations for a Rapid and High Throughput Measurement of Catalase In Vitro

• Main Authors: Ouardia Bendou (Author), Ismael Gutiérrez-Fernández (Author), Emilio L. Marcos-Barbero (Author), Nara Bueno-Ramos (Author), Ana I. González-Hernández (Author), Rosa Morcuende (Author), Juan B. Arellano (Author)
• Format: Book
• Published: MDPI AG, 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A rapid and high throughput protocol to measure the catalase activity in vitro has been designed. Catalase is an enzyme with unusual kinetic properties because it does not follow the standard Michaelis-Menten model and is inactivated by H₂O₂. This makes...