TREX2 enables efficient genome disruption mediated by paired CRISPR-Cas9 nickases that generate 3'-overhanging ends

• Main Authors: Yue Wang (Author), Yi-Li Feng (Author), Qian Liu (Author), Jing-Jing Xiao (Author), Si-Cheng Liu (Author), Zhi-Cheng Huang (Author), An-Yong Xie (Author)
• Format: Book
• Published: Elsevier, 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Paired SpCas9 nickases (SpCas9n) are an effective strategy to reduce off-target effect in genome editing. However, this approach is not efficient with 3'-overhanging ends, limiting its applications. In order to expand the utility of paired SpCas9n in genome editing, we tested the effect of the...