Direct in vitro and in vivo demonstration of muscarinic receptor binding by the novel radioligand, [3H]5-hydroxymethyltolterodine, in the bladder and other tissues of rats

• Main Authors: Shizuo Yamada (Author), Shiori Kuraoka (Author), Yoshihiko Ito (Author), Satomi Kagota (Author), Kazumasa Shinozuka (Author), Satomi Onoue (Author)
• Format: Book
• Published: Elsevier, 2020-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In vitro and in vivo binding sites of [3H]-labeled 5-hydroxymethyltolterodine (5-HMT), a new radioligand for labeling muscarinic receptors in rat tissues were characterized. Specific [3H]5-HMT binding in rat tissues was saturable and of high affinity in each tissue. The dissociation constant (Kd) wa...