Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing.

• Päätekijät: Kyoko Hayashida (Tekijä), Yasuko Orba (Tekijä), Patricia C Sequeira (Tekijä), Chihiro Sugimoto (Tekijä), William W Hall (Tekijä), Yuki Eshita (Tekijä), Yutaka Suzuki (Tekijä), Lucky Runtuwene (Tekijä), Patricia Brasil (Tekijä), Guilherme Calvet (Tekijä), Cintia D S Rodrigues (Tekijä), Carolina C Dos Santos (Tekijä), Maria A M Mares-Guia (Tekijä), Junya Yamagishi (Tekijä), Ana M B de Filippis (Tekijä), Hirofumi Sawa (Tekijä)
• Aineistotyyppi: Kirja
• Julkaistu: Public Library of Science (PLoS), 2019-06-01T00:00:00Z.
• Aiheet: article
• Linkit: Connect to this object online.

Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer. We developed the protocol for drying all the reagents for the RT-LAMP in a single reactio...