Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing.

• Main Authors: Kyoko Hayashida (Author), Yasuko Orba (Author), Patricia C Sequeira (Author), Chihiro Sugimoto (Author), William W Hall (Author), Yuki Eshita (Author), Yutaka Suzuki (Author), Lucky Runtuwene (Author), Patricia Brasil (Author), Guilherme Calvet (Author), Cintia D S Rodrigues (Author), Carolina C Dos Santos (Author), Maria A M Mares-Guia (Author), Junya Yamagishi (Author), Ana M B de Filippis (Author), Hirofumi Sawa (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer. We developed the protocol for drying all the reagents for the RT-LAMP in a single reactio...