Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing.

• Главные авторы: Kyoko Hayashida (Автор), Yasuko Orba (Автор), Patricia C Sequeira (Автор), Chihiro Sugimoto (Автор), William W Hall (Автор), Yuki Eshita (Автор), Yutaka Suzuki (Автор), Lucky Runtuwene (Автор), Patricia Brasil (Автор), Guilherme Calvet (Автор), Cintia D S Rodrigues (Автор), Carolina C Dos Santos (Автор), Maria A M Mares-Guia (Автор), Junya Yamagishi (Автор), Ana M B de Filippis (Автор), Hirofumi Sawa (Автор)
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• Опубликовано: Public Library of Science (PLoS), 2019-06-01T00:00:00Z.
• Предметы: article
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Detection and sequencing of chikungunya virus (CHIKV) genome was performed using a combination of a modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and a MinION sequencer. We developed the protocol for drying all the reagents for the RT-LAMP in a single reactio...