Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

• Main Authors: Noriaki Kaneko (Author), Tetsuya Rikimaru (Author), Tetsuyuki Fujimura (Author), Shigeyasu Mori (Author), Saburo Hidaka (Author), Hidehiro Kaya (Author)
• Format: Book
• Published: Hindawi Limited, 2010-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifu...