Determining the affinities of high-affinity antibodies using KinExA and surface plasmon resonance

• Main Authors: M. Frank Erasmus (Author), Molly Dovner (Author), Fortunato Ferrara (Author), Sara D'Angelo (Author), André A. Teixeira (Author), Camila Leal-Lopes (Author), Laura Spector (Author), Elizabeth Hopkins (Author), Andrew R. M. Bradbury (Author)
• Format: Book
• Published: Taylor & Francis Group, 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

ABSTRACTAccurate and efficient affinity measurement techniques are essential for the biophysical characterization of therapeutic monoclonal antibodies, one of the fastest growing drug classes. Surface plasmon resonance (SPR) is widely used for determining antibody affinity, but does not perform well...