Comparative production analysis of three phlebovirus nucleoproteins under denaturing or non-denaturing conditions for crystallographic studies.

• Main Authors: Violaine Lantez (Author), Karen Dalle (Author), Rémi Charrel (Author), Cécile Baronti (Author), Bruno Canard (Author), Bruno Coutard (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2011-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Nucleoproteins (NPs) encapsidate the Phlebovirus genomic (-)RNA. Upon recombinant expression, NPs tend to form heterogeneous oligomers impeding characterization of the encapsidation process through crystallographic studies. To overcome this problem, we set up a standard protocol in which production...