Comparative production analysis of three phlebovirus nucleoproteins under denaturing or non-denaturing conditions for crystallographic studies.

• প্রধান লেখক: Violaine Lantez (লেখক), Karen Dalle (লেখক), Rémi Charrel (লেখক), Cécile Baronti (লেখক), Bruno Canard (লেখক), Bruno Coutard (লেখক)
• বিন্যাস: গ্রন্থ
• প্রকাশিত: Public Library of Science (PLoS), 2011-01-01T00:00:00Z.
• বিষয়গুলি: article
• অনলাইন ব্যবহার করুন: Connect to this object online.

Nucleoproteins (NPs) encapsidate the Phlebovirus genomic (-)RNA. Upon recombinant expression, NPs tend to form heterogeneous oligomers impeding characterization of the encapsidation process through crystallographic studies. To overcome this problem, we set up a standard protocol in which production...