Hydrophobically Modified siRNAs Silence Huntingtin mRNA in Primary Neurons and Mouse Brain

• Main Authors: Julia F Alterman (Author), Lauren M Hall (Author), Andrew H Coles (Author), Matthew R Hassler (Author), Marie-Cecile Didiot (Author), Kathryn Chase (Author), Jasmin Abraham (Author), Emily Sottosanti (Author), Emily Johnson (Author), Ellen Sapp (Author), Maire F Osborn (Author), Marian Difiglia (Author), Neil Aronin (Author), Anastasia Khvorova (Author)
• Format: Book
• Published: Elsevier, 2015-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Applications of RNA interference for neuroscience research have been limited by a lack of simple and efficient methods to deliver oligonucleotides to primary neurons in culture and to the brain. Here, we show that primary neurons rapidly internalize hydrophobically modified siRNAs (hsiRNAs) added di...