Enhanced sensitivity of neutralizing antibody detection for different AAV serotypes using HeLa cells with overexpressed AAVR

• Main Authors: Zhaoyue Zheng (Author), Jingya Ye (Author), Mi Leng (Author), Chunmei Gan (Author), Na Tang (Author), Wei Li (Author), C. Alexander Valencia (Author), Biao Dong (Author), Hoi Yee Chow (Author)
• Format: Book
• Published: Frontiers Media S.A., 2023-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A cell-based transduction inhibition assay (TI) is widely used in clinical trials to detect neutralizing antibody (NAb) titers against recombinant adeno-associated virus (rAAV), one of the most important criteria to exclude patients in gene therapy. Different cell lines are used in cell-based TI bec...