Duplex real-time PCR for sexing Schistosoma japonicum cercariae based on W chromosome-specific genes and its applications.

• Main Authors: Shuai Liu (Author), Xianyu Piao (Author), Nan Hou (Author), Pengfei Cai (Author), Yu Ma (Author), Qijun Chen (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2020-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

As a unique feature among otherwise hermaphroditic trematodes, Schistosoma species are gonochoric parasites whose sex is genetically determined (ZZ for males and ZW for females). However, schistosome larvae are morphologically identical, and sex can only be discriminated by molecular methods. Here,...